GPR158 Polyclonal antibody

• 

  WB analysis using 31846-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31846-1-AP (GPR158 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GPR158 Polyclonal Antibody for WB, ELISA

Cat No. 31846-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Probable G-Protein Coupled Receptor 158, mGlyR, Metabotropic glycine receptor, KIAA1136, G-protein coupled receptor 158

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31846-1-AP targets GPR158 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR158 fusion protein Ag34602
• Full Name: G protein-coupled receptor 158
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC157124
• Gene Symbol: GPR158
• Gene ID (NCBI): 57512
• RRID: AB_3670127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q5T848
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

G protein-coupled receptors (GPCR) are the largest family of proteins encoded in mammalian genomes that detect extracellular signals to program cellular response, which are essential to understanding physiology, disease, and drug development (PMID: 29249361; 29298813). G protein-coupled receptor 158 (GPR158, also known as mGlyR) is a class C orphan GPCR prominently expressed in brain tissue, where it controls synapse formation and function (PMID: 22689652; 34793198). It can transduce signals by coupling to the regulator of protein signaling (RGS) proteins and be related to the development of major depressive disorder and prostate cancer (PMID: 36996198; 29419376; 25693195).