GPNMB Recombinant antibody, PBS Only (Capture)

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  WB analysis of THP-1 using 84160-3-RR (same clone as 84160-3-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

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  WB analysis using 84160-3-RR (same clone as 84160-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84160-3-RR (GPNMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01096-1

  Sandwich ELISA standard curve of MP01096-1, Human GPNMB Recombinant Matched Antibody Pair - PBS only. 84160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14124. 84160-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

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  Sample test of MP01096-1

  Plasma of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 12.18 ng/mL with a range of 3.43-30.61 ng/mL.

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  Sample test of MP01096-1

  Serum of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 5.69 ng/mL with a range of 3.96-8.17 ng/mL.

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  IF Staining of HeLa using 84160-3-RR (same clone as 84160-3-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (84160-3-RR, Clone: 241435F3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84160-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01096-1

  Cytometric bead array standard curve of MP01096-1, GPNMB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84160-3-PBS. Detection antibody: 84160-2-PBS. Standard: Ag14124. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84160-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84160-3-RR against Human GPNMB were performed. The affinity constant is below 1 pM.

GPNMB Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84160-3-PBS

Clone No.241435F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

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Product Information

84160-3-PBS targets GPNMB as part of a matched antibody pair:

MP01096-1: 84160-3-PBS capture and 84160-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPNMB fusion protein Ag14124
• Full Name: glycoprotein (transmembrane) nmb
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 120 kDa, 95 kDa
• GenBank Accession Number: BC011595
• Gene Symbol: GPNMB
• Gene ID (NCBI): 10457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14956
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.