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GPNMB Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 0.781-50 ng/mL

Cat No : MP01096-1

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01096-1, Human GPNMB Recombinant Matched Antibody Pair - PBS only. 84160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14124. 84160-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

    Sandwich ELISA standard curve of MP01096-1

    Sandwich ELISA standard curve of MP01096-1, Human GPNMB Recombinant Matched Antibody Pair - PBS only. 84160-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14124. 84160-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.

  • Serum of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 5.69 ng/mL with a range of 3.96-8.17 ng/mL.

    Sample test of MP01096-1

    Serum of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 5.69 ng/mL with a range of 3.96-8.17 ng/mL.

  • Plasma of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 12.18 ng/mL with a range of 3.43-30.61 ng/mL.

    Sample test of MP01096-1

    Plasma of six individual healthy human donors was measured. The GPNMB concentration of detected samples was determined to be 12.18 ng/mL with a range of 3.43-30.61 ng/mL.

  • Cytometric bead array standard curve of MP01096-1, GPNMB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84160-3-PBS. Detection antibody: 84160-2-PBS. Standard: Ag14124. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP01096-1

    Cytometric bead array standard curve of MP01096-1, GPNMB Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84160-3-PBS. Detection antibody: 84160-2-PBS. Standard: Ag14124. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01096-1 targets GPNMB in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 0.781-50 ng/mL (Sandwich ELISA);

Capture antibody: GPNMB Recombinant antibody, PBS Only (Capture) 84160-3-PBS (241435F3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: GPNMB Recombinant antibody, PBS Only (Detector) 84160-2-PBS (241435D10). 100 μg. Concentration 1 mgl/ml.

Alternative GPNMB matched antibody pairs: MP01096-2, MP50045-1, MP50045-2

Matched pair protein standard: Ag14124

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84160-3-PBS
Tested Reactivity human
Immunogen GPNMB fusion protein Ag14124
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type
GenBank accession numberBC011595
Gene symbol GPNMB
UniprotQ14956
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84160-2-PBS
Tested Reactivity human
Immunogen GPNMB fusion protein Ag14124
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type
GenBank accession numberBC011595
Gene symbol GPNMB
UniprotQ14956
Gene ID (NCBI) 10457
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.