GPAT3 Polyclonal antibody, PBS Only

GPAT3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30765-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30765-1-AP (same clone as 30765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30765-1-AP (same clone as 30765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-1080 using 30765-1-AP (same clone as 30765-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 30765-1-AP (same clone as 30765-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 30765-1-AP (same clone as 30765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30765-1-AP (same clone as 30765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 30765-1-AP (same clone as 30765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 30765-1-AP (same clone as 30765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30765-1-PBS in a different storage buffer formulation.

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Product Information

30765-1-PBS targets GPAT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPAT3 fusion protein Ag33717
• Full Name: 1-acylglycerol-3-phosphate O-acyltransferase 9
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: Glycerol-3-phosphate acyltransferase 3
• Gene Symbol: AGPAT9
• Gene ID (NCBI): 84803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.