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GPAT3 Polyclonal antibody

GPAT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30765-1-AP

Synonyms

AGPAT9, 1-AGPAT 9, 1-AGP acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis using 30765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis using 30765-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis of HT-1080 using 30765-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GPAT3 Polyclonal antibody (30765-1-AP)

    WB analysis of HeLa using 30765-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30765-1-AP (GPAT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse liver using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse liver using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse kidney using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using GPAT3 Polyclonal antibody (30765-1-AP)

    IHC staining of mouse kidney using 30765-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30765-1-AP (GPAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, HT-1080 cells, A375 cells, Jurkat cells, MKN-45 cells
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30765-1-AP targets GPAT3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPAT3 fusion protein Ag33717
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 9
Calculated Molecular Weight49 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberGlycerol-3-phosphate acyltransferase 3
Gene Symbol AGPAT9
Gene ID (NCBI) 84803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.

Protocols

Product Specific Protocols
WB protocol for GPAT3 antibody 30765-1-APDownload protocol
IHC protocol for GPAT3 antibody 30765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols