GPAA1 Recombinant antibody

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  WB analysis using 84632-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84632-1-RR (GPAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84632-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84632-1-RR against Human GPAA1 were performed. The affinity constant is 0.141 nM.

GPAA1 Recombinant Antibody for WB, ELISA

Cat No. 84632-1-RR

Clone No.241591C4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hGAA1, GPAA 1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1, 241591C4

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Tested Applications

• Positive WB detected in: MKN-45 cells, HeLa cells, HepG2 cells, HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84632-1-RR targets GPAA1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPAA1 fusion protein Ag0152
• Full Name: glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 60-68 kDa
• GenBank Accession Number: BC004129
• Gene Symbol: GPAA1
• Gene ID (NCBI): 8733
• RRID: AB_3672093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43292
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837).