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GPAA1 Recombinant antibody, PBS Only

GPAA1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241591C4

Cat no : 84632-1-PBS

Synonyms

hGAA1, GPAA 1, Glycosylphosphatidylinositol anchor attachment 1 protein, GAA1, 241591C4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPAA1 Recombinant antibody (84632-1-RR)

    WB analysis using 84632-1-RR (same clone as 84632-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84632-1-RR (GPAA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84632-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84632-1-RR

    Affinity and Kinetic Characterization of 84632-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84632-1-RR against Human GPAA1 were performed. The affinity constant is 0.141 nM.

Product Information

The immunogen of 84632-1-PBS is GPAA1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPAA1 fusion protein Ag0152
Full Name glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 60-68 kDa
GenBank Accession NumberBC004129
Gene Symbol GPAA1
Gene ID (NCBI) 8733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837).