GP73/GOLPH2 Recombinant antibody, PBS Only

GP73/GOLPH2 Recombinant Antibody for WB, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I12

Cat no : 81893-1-PBS

Synonyms

bA379P1.3, C9orf155, FLJ22634, FLJ23608, Golgi marker, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, PSEC0257, UNQ686/PRO1326

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Validation Data Gallery

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  WB analysis using 81893-1-RR (same clone as 81893-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:450 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 81893-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81893-1-PBS targets GP73/GOLPH2 in WB, IF, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GP73/GOLPH2 fusion protein Ag7207
• Full Name: golgi membrane protein 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001740
• Gene Symbol: GP73
• Gene ID (NCBI): 51280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.