Filter: AllWBIHCIF/ICCAffinity

WB analysis using 81893-1-RR (same clone as 81893-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 81893-1-RR (same clone as 81893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IHC staining of human ovarian cancer using 81893-1-RR (same clone as 81893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 81893-1-RR (same clone as 81893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IHC staining of human stomach using 81893-1-RR (same clone as 81893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:450 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 81893-1-RR
Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

GP73/GOLPH2 Recombinant monoclonal antibody, PBS Only

GP73/GOLPH2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 81893-1-PBS

Clone No.1I12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GOLM1, GOLPH2, GP73, 1I12, C9orf155

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

81893-1-PBS targets GP73/GOLPH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag7207 Product name: Recombinant human GOLM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-400 aa of BC001740 Sequence: SRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL Predict reactive species
Full Name	golgi membrane protein 1
Calculated Molecular Weight	45 kDa
Observed Molecular Weight	70-75 kDa
GenBank Accession Number	BC001740
Gene Symbol	GP73
Gene ID (NCBI)	51280
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8NBJ4
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.