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GP73/GOLPH2 Recombinant antibody

GP73/GOLPH2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1I12

Cat no : 81893-1-RR

Synonyms

GOLM1, GOLPH2, GP73, golgi protein 73kD, Golgi phosphoprotein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    WB analysis using 81893-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human ovarian cancer using 81893-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human stomach using 81893-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human stomach cancer using 81893-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human stomach cancer using 81893-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IF Staining of HeLa using 81893-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:450 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IF Staining of HeLa using 81893-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81893-1-RR

    Affinity and Kinetic Characterization of 81893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue, human ovarian cancer, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:450-1:1800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81893-1-RR targets GP73/GOLPH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207
Full Name golgi membrane protein 1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC001740
Gene Symbol GP73
Gene ID (NCBI) 51280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for GP73/GOLPH2 antibody 81893-1-RRDownload protocol
IHC protocol for GP73/GOLPH2 antibody 81893-1-RRDownload protocol
IF protocol for GP73/GOLPH2 antibody 81893-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols