CoraLite® Plus 488-conjugated CD42b Monoclonal antibody

CD42b Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G1F5

Cat no : CL488-67857

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA

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Validation Data Gallery

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  WB analysis using CL488-67857

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with CL488-67857 (CD42b antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human peripheral blood platelets

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67857 targets CD42b in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD42b fusion protein Ag31181
• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 626 aa, 69 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC027955
• Gene Symbol: CD42b
• Gene ID (NCBI): 2811
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD42b, also named as GP1BA , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.