CoraLite® Plus 488-conjugated GOT2 Monoclonal antibody

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  FC experiment of HeLa using CL488-67738

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GOT2 (CL488-67738, Clone:1B6C6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GOT2 Monoclonal Antibody for FC (Intra)

Cat No. CL488-67738

Clone No.1B6C6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, chicken

Applications

FC (Intra)

Synonyms

FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial, AATM

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67738 targets GOT2 in FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOT2 fusion protein Ag6600
• Full Name: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 39-40 kDa
• GenBank Accession Number: BC000525
• Gene Symbol: GOT2
• Gene ID (NCBI): 2806
• RRID: AB_2923785
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P00505
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.