GOT2 Monoclonal antibody, PBS Only

GOT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6C6

Cat no : 67738-1-PBS

Synonyms

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

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Validation Data Gallery

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  WB analysis using 67738-1-Ig (same clone as 67738-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67738-1-Ig (same clone as 67738-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 67738-1-Ig (same clone as 67738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 67738-1-Ig (same clone as 67738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67738-1-Ig (same clone as 67738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 67738-1-Ig (same clone as 67738-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67738-1-PBS targets GOT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.

• Tested Reactivity: Human, mouse, rat, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOT2 fusion protein Ag6600
• Full Name: glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 39-40 kDa
• GenBank Accession Number: BC000525
• Gene Symbol: GOT2
• Gene ID (NCBI): 2806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.