CoraLite®594-conjugated GOT1 Monoclonal antibody

GOT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A12E10

Cat no : CL594-60317

Synonyms

CAT, GIG18, GOT1, Transaminase A

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-60317

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-60317

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 GOT1 antibody (CL594-60317, Clone: 5A12E10 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60317 targets GOT1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOT1 fusion protein Ag7012
• Full Name: glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-46 kDa
• GenBank Accession Number: BC000498
• Gene Symbol: GOT1
• Gene ID (NCBI): 2805
• RRID: AB_2883463
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.