GORASP2 Monoclonal antibody, PBS Only

GORASP2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9A3

Cat no : 66627-1-PBS

Synonyms

Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

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Validation Data Gallery

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  WB analysis using 66627-1-Ig (same clone as 66627-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66627-1-Ig (same clone as 66627-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66627-1-Ig (same clone as 66627-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66627-1-Ig (same clone as 66627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66627-1-Ig (same clone as 66627-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66627-1-Ig (same clone as 66627-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (66627-1-Ig, Clone: 1C9A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66627-1-PBS targets GORASP2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig samples.

• Tested Reactivity: Human, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GORASP2 fusion protein Ag17607
• Full Name: golgi reassembly stacking protein 2, 55kDa
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC007770
• Gene Symbol: GORASP2
• Gene ID (NCBI): 26003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.