GORASP2 Monoclonal antibody
GORASP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Pig and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1C9A3
Cat no : 66627-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, pig brain tissue, rat brain tissue, NCCIT cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 4 publications below |
Product Information
66627-1-Ig targets GORASP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Pig samples.
| Tested Reactivity | Human, Rat, Pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GORASP2 fusion protein Ag17607 |
| Full Name | golgi reassembly stacking protein 2, 55kDa |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC007770 |
| Gene Symbol | GORASP2 |
| Gene ID (NCBI) | 26003 |
| RRID | AB_2881987 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GORASP2 antibody 66627-1-Ig | Download protocol |
| IHC protocol for GORASP2 antibody 66627-1-Ig | Download protocol |
| IF protocol for GORASP2 antibody 66627-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. | ||
J Cell Biol Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure. | ||
Front Cell Dev Biol Long-term culture of patient-derived cardiac organoids recapitulated Duchenne muscular dystrophy cardiomyopathy and disease progression | ||
Cell Death Dis A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection. | ||
Cell Commun Signal CHOP upregulation and dysregulation of the mature form of the SNAT2 amino acid transporter in the placentas from small for gestational age newborns |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (01-11-2020) | This antibody can be used for detecting the Golgi localisation of endogenous Gorasp2.
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