GOLIM4 Recombinant antibody

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  WB analysis using 84818-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84818-5-RR (GOLIM4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 84818-5-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GOLIM4 antibody (84818-5-RR, Clone: 242323F4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-2 OS using 84818-5-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HeLa using 84818-5-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GOLIM4 Recombinant antibody (84818-5-RR, Clone:242323F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84818-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84818-5-RR against Human GOLIM4 were performed. The affinity constant is 0.926 nM.

GOLIM4 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 84818-5-RR

Clone No.242323F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

P138, P 138, GPP130, GOLPH4, GIMPC

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, HT-1080 cells, L02 cells, C2C12 cells
• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: U-2 OS, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84818-5-RR targets GOLIM4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GOLIM4 fusion protein Ag34962
• Full Name: golgi integral membrane protein 4
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_014498
• Gene Symbol: GOLIM4
• Gene ID (NCBI): 27333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O00461
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).