Filter: AllWBIHCIF/ICCFC (Intra)Affinity

WB analysis using 83009-4-RR (same clone as 83009-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 83009-4-RR (same clone as 83009-4-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83009-4-RR (golgin 97 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83009-4-RR
Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

golgin 97 Recombinant antibody, PBS Only

golgin 97 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83009-4-PBS

Clone No.230523A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83009-4-PBS targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	golgin 97 fusion protein Ag3335 Predict reactive species
Full Name	golgi autoantigen, golgin subfamily a, 1
Calculated Molecular Weight	767 aa, 88 kDa
Observed Molecular Weight	97 kDa
GenBank Accession Number	BC032853
Gene Symbol	golgin 97
Gene ID (NCBI)	2800
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q92805
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.