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golgin 97 Recombinant antibody

golgin 97 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230523A5

Cat no : 83009-4-RR

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using golgin 97 Recombinant antibody (83009-4-RR)

    WB analysis using 83009-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using golgin 97 Recombinant antibody (83009-4-RR)

    IHC staining of human ovary cancer using 83009-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83009-4-RR (golgin 97 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using golgin 97 Recombinant antibody (83009-4-RR)

    IF Staining of HepG2 using 83009-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using golgin 97 Recombinant antibody (83009-4-RR)

    FC experiment of HepG2 using 83009-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83009-4-RR

    Affinity and Kinetic Characterization of 83009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, LNCaP cells, MCF-7 cells, SMMC-7721 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83009-4-RR is golgin 97 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen golgin 97 fusion protein Ag3335
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated Molecular Weight 767 aa, 88 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC032853
Gene Symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.

Protocols

Product Specific Protocols
WB protocol for golgin 97 antibody 83009-4-RRDownload protocol
IHC protocol for golgin 97 antibody 83009-4-RRDownload protocol
IF protocol for golgin 97 antibody 83009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols