golgin 97 Recombinant antibody, PBS Only

golgin 97 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230523A5

Cat no : 83009-4-PBS

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

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Validation Data Gallery

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  WB analysis using 83009-4-RR (same clone as 83009-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83009-4-RR (same clone as 83009-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83009-4-RR (golgin 97 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83009-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83009-4-PBS is golgin 97 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: golgin 97 fusion protein Ag3335
• Full Name: golgi autoantigen, golgin subfamily a, 1
• Calculated Molecular Weight: 767 aa, 88 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC032853
• Gene Symbol: golgin 97
• Gene ID (NCBI): 2800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.