GNMT Polyclonal antibody, PBS Only

GNMT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18790-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of rat liver using 18790-1-AP (same clone as 18790-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate hyperplasia using 18790-1-AP (same clone as 18790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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Product Information

18790-1-PBS targets GNMT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNMT fusion protein Ag4598
• Full Name: glycine N-methyltransferase
• Calculated Molecular Weight: 295 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC032627
• Gene Symbol: GNMT
• Gene ID (NCBI): 27232
• RRID: AB_10597093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).