GNMT Polyclonal antibody

GNMT Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 18790-1-AP

Synonyms

glycine N methyltransferase, GNMT

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Validation Data Gallery

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  WB analysis using 18790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 18790-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18790-1-AP

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.

• 

  IP experiment of mouse liver using 18790-1-AP

  IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human prostate hyperplasia using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 18790-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat liver tissue, mouse liver tissue, LNCaP cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human prostate hyperplasia tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 2 publications below

Product Information

18790-1-AP targets GNMT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNMT fusion protein Ag4598
• Full Name: glycine N-methyltransferase
• Calculated Molecular Weight: 295 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC032627
• Gene Symbol: GNMT
• Gene ID (NCBI): 27232
• RRID: AB_10597093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

Publications

Species	Application	Title
mouse	WB	Mol Metab; Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction.; Authors - Ferrol I Rome
human	IHC	Int J Mol Sci; MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients.; Authors - Pei-Yi Chu
	WB	J Anim Sci Biotechnol; Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression.; Authors - Nagmeldin A Omer
human	WB	Mol Oncol; Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.; Authors - Jie Li
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang
pig	WB	J Agric Food Chem; Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.; Authors - Yimin Jia