CoraLite®594-conjugated GNAS Monoclonal antibody

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  IF Staining of human colon cancer using CL594-66253

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-66253

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

• 

  IF Staining of human colon cancer using CL594-66253

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

• 

  IF Staining of MCF-7 using CL594-66253

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 GNAS antibody (CL594-66253, Clone: 1A9C10 ) at dilution of 1:200.

GNAS Monoclonal Antibody for IF/ICC, IF-P

Cat No. CL594-66253

Clone No.1A9C10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Synonyms

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66253 targets GNAS in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNAS fusion protein Ag20530
• Full Name: GNAS complex locus
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC002722
• Gene Symbol: GNAS
• Gene ID (NCBI): 2778
• RRID: AB_2919971
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95467
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.