GNAO1 Monoclonal antibody
GNAO1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Mouse, Rat, Rabbit, Pig, Chicken, Human
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1B5G2
Cat no : 68027-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rat cerebellum tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat cerebellum tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68027-1-Ig targets GNAO1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Mouse, Rat, Rabbit, Pig, Chicken, Human samples.
Tested Reactivity | Mouse, Rat, Rabbit, Pig, Chicken, Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | GNAO1 fusion protein Ag32045 |
Full Name | guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O |
Calculated Molecular Weight | 302 aa, 35 kDa |
Observed Molecular Weight | 40 kDa |
GenBank Accession Number | BC030027 |
Gene Symbol | GNAO1 |
Gene ID (NCBI) | 2775 |
RRID | AB_2918770 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for GNAO1 antibody 68027-1-Ig | Download protocol |
IHC protocol for GNAO1 antibody 68027-1-Ig | Download protocol |
IF protocol for GNAO1 antibody 68027-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |