GNAI1 Monoclonal antibody, PBS Only

GNAI1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Mouse, Rat, Pig, Chicken

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G11H5

Cat no : 68266-1-PBS

Synonyms

Gi, GNAI1

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Validation Data Gallery

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  WB analysis using 68266-1-Ig (same clone as 68266-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68266-1-PBS in a different storage buffer formulation.

• 

  WB analysis of chicken brain using 68266-1-Ig (same clone as 68266-1-PBS)

  chicken brain tissue were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68266-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 68266-1-Ig (same clone as 68266-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68266-1-PBS in a different storage buffer formulation.

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Product Information

68266-1-PBS targets GNAI1 in WB, Indirect ELISA applications and shows reactivity with Mouse, Rat, Pig, Chicken samples.

• Tested Reactivity: Mouse, Rat, Pig, Chicken
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNAI1 fusion protein Ag3290
• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC026326
• Gene Symbol: GNAI1
• Gene ID (NCBI): 2770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.