GNAI1 Monoclonal antibody

GNAI1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Mouse, Rat, Pig, Chicken

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G11H5

Cat no : 68266-1-Ig

Synonyms

Gi, GNAI1

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Validation Data Gallery

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  WB analysis using 68266-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of chicken brain using 68266-1-Ig

  chicken brain tissue were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 68266-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat brain tissue, pig brain tissue, chicken brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68266-1-Ig targets GNAI1 in WB, ELISA applications and shows reactivity with Mouse, Rat, Pig, Chicken samples.

• Tested Reactivity: Mouse, Rat, Pig, Chicken
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GNAI1 fusion protein Ag3290
• Full Name: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
• Calculated Molecular Weight: 354 aa, 40 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC026326
• Gene Symbol: GNAI1
• Gene ID (NCBI): 2770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.