CoraLite®594-conjugated GMNN Monoclonal antibody

GMNN Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E3A2

Cat no : CL594-66566

Synonyms

Gem, Geminin, GMNN, RP3 369A17.3

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66566

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GMNN antibody (CL594-66566, Clone: 2E3A2 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-66566

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 GMNN antibody (CL594-66566, Clone: 2E3A2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66566 targets GMNN in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GMNN fusion protein Ag24283
• Full Name: geminin, DNA replication inhibitor
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005185
• Gene Symbol: GMNN
• Gene ID (NCBI): 51053
• RRID: AB_2920007
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)