Geminin Polyclonal antibody

• Featured Product
• KD/KO Validated

Geminin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

59

Cat no : 10802-1-AP

Synonyms

GMNN, MGORS6, Gem

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Validation Data Gallery

• 

  WB analysis using 10802-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10802-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10802-1-AP

  WB result of Geminin antibody (10802-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Geminin transfected HEK-293 cells.

• 

  WB analysis of mouse testis using 10802-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10802-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10802-1-AP (Geminin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10802-1-AP

  IP result of anti-Geminin (IP:10802-1-AP, 4ug; Detection:10802-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human colon cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10802-1-AP (Geminin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human breast cancer using 10802-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10802-1-AP (Geminin antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 10802-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (10802-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 10802-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of HeLa using 10802-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Geminin (10802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue, mouse testis tissue, HeLa cells; mouse testis tissue, rat testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 12 publications below
• IF: See 39 publications below
• IP: See 1 publications below

Product Information

10802-1-AP targets Geminin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Geminin fusion protein Ag1076
• Full Name: geminin, DNA replication inhibitor
• Calculated Molecular Weight: 24 kDa
• Observed Molecular Weight: 28-29 kDa
• GenBank Accession Number: BC005185
• Gene Symbol: GMNN
• Gene ID (NCBI): 51053
• RRID: AB_2110945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)

Publications

Species	Application	Title
mouse	IF	Nature; Whole genomes redefine the mutational landscape of pancreatic cancer.; Authors - Nicola Waddell
human	IF	Cancer Discov; Biomarkers Associating with PARP Inhibitor Benefit in Prostate Cancer in the TOPARP-B Trial.; Authors - Suzanne Carreira
human	IF	Ann Oncol; Association of RAD51 with homologous recombination deficiency (HRD) and clinical outcomes in untreated triple-negative breast cancer (TNBC): analysis of the GeparSixto randomized clinical trial.; Authors - A Llop-Guevara
human	WB	Nat Commun; DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation; Authors - Xiaohua Xu
mouse	IF	Nat Commun; Homologous recombination deficiency derived from whole-genome sequencing predicts platinum response in triple-negative breast cancers; Authors - Petra Ter Brugge
human	IF	Sci Transl Med; BRCAness, SLFN11, and RB1 loss predict response to topoisomerase I inhibitors in triple-negative breast cancers.; Authors - Florence Coussy

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