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CoraLite® Plus 488-conjugated GMF Beta Monoclonal antibody

GMF Beta Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B10A2

Cat no : CL488-60062

Synonyms

Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using CoraLite® Plus 488-conjugated GMF Beta Monoclonal (CL488-60062)

    IF Staining of U-87 MG using CL488-60062

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using CoraLite® Plus 488 GMF Beta antibody (CL488-60062, Clone: 3B10A2 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60062 targets GMF Beta in IF/ICC applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMF Beta fusion protein Ag1104
Full Name glia maturation factor, beta
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC005359
Gene Symbol GMFB
Gene ID (NCBI) 2764
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GMF Beta antibody CL488-60062Download protocol
Standard Protocols
Click here to view our Standard Protocols