CoraLite® Plus 488-conjugated GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7A8

Cat no : CL488-68242

Synonyms

GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68242

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 GLO1 antibody (CL488-68242, Clone: 1B7A8 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-68242

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Glo1 Monoclonal Antibody (CL488-68242, Clone:1B7A8)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68242 targets GLO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLO1 fusion protein Ag7314
• Full Name: glyoxalase I
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: BC001741
• Gene Symbol: GLO1
• Gene ID (NCBI): 2739
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.