GLI1 Monoclonal antibody, PBS Only

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 66905-1-Ig (same clone as 66905-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66905-1-Ig (same clone as 66905-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLI1 antibody (66905-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GLI1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66905-1-PBS

Clone No.1D2B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Oncogene GLI, Glioma-associated oncogene, Glioma associated oncogene, GLI family zinc finger 1, GLI

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Product Information

66905-1-PBS targets GLI1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GLI1 fusion protein Ag27832
• Full Name: GLI family zinc finger 1
• Calculated Molecular Weight: 1106 aa, 118 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC013000
• Gene Symbol: GLI1
• Gene ID (NCBI): 2735
• RRID: AB_2882232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08151
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GLI1, also known as Glioma-associated oncogene, is a 1106 amino acid protein, which belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development (PubMed:19706761). GLI1 plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract and Mediates SHH signaling (PubMed:19706761). GLI1 plays a role in cell proliferation and differentiation via its role in SHH signaling.