Biotin Plus-conjugated GLAST/EAAT1 Polyclonal antibody

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  IHC staining of mouse brain using Biotin-20785

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-20785 (GLAST/EAAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using Biotin-20785

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using Biotin-20785 (GLAST/EAAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

GLAST/EAAT1 Polyclonal Antibody for IHC

Cat No. Biotin-20785

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

GLAST, EAAT1, Sodium-dependent glutamate/aspartate transporter 1, SLC1A3, GLAST-1

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Tested Applications

• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-20785 targets GLAST/EAAT1 in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLAST/EAAT1 fusion protein Ag14177
• Full Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Calculated Molecular Weight: 542 aa, 60 kDa
• Observed Molecular Weight: 50-55 kDa, 90-100 kDa
• GenBank Accession Number: BC037310
• Gene Symbol: GLAST
• Gene ID (NCBI): 6507
• Conjugate: Biotin Plus
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43003
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)