GLAST Polyclonal antibody

• Featured Product
• KD/KO Validated

GLAST Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Reviews (2)

Cat no : 20785-1-AP

Synonyms

EAAT1, Sodium-dependent glutamate/aspartate transporter 1, SLC1A3, GLAST-1

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Validation Data Gallery

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  WB analysis of Neuro-2a using 20785-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 20785-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20785-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20785-1-AP (GLAST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20785-1-AP

  IP result of anti-GLAST (IP:20785-1-AP, 4ug; Detection:20785-1-AP 1:500) with mouse brain tissue lysate 3000ug.

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  IHC staining of human brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 20785-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20785-1-AP (GLAST antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of Neuro-2a using 20785-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using GLAST antibody (20785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Neuro-2a using 20785-1-AP

  1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human GLAST (20785-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, C6 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Neuro-2a cells
• Positive FC (Intra) detected in: Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

20785-1-AP targets GLAST in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, cow
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLAST fusion protein Ag14177
• Full Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Calculated Molecular Weight: 542 aa, 60 kDa
• Observed Molecular Weight: 50-55 kDa, 90-100 kDa
• GenBank Accession Number: BC037310
• Gene Symbol: GLAST
• Gene ID (NCBI): 6507
• RRID: AB_2878738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)

Publications

Species	Application	Title
mouse	IF	Nucleic Acids Res; H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.; Authors - Libo Su
mouse		Cell Rep; SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice; Authors - Yan Wang
mouse	WB, IF	Cell Death Differ; RNF20 controls astrocytic differentiation through epigenetic regulation of STAT3 in the developing brain.; Authors - Qingli Liang
human	WB	J Neuroimmune Pharmacol; Brain-Derived Exosomal CircRNAs in Plasma Serve as Diagnostic Biomarkers for Acute Ischemic Stroke; Authors - Xinli Jiang
mouse	WB	Neuropharmacology; Ginsenoside Rb1 confers neuroprotection via promotion of glutamate transporters in a mouse model of Parkinson's disease.; Authors - Yun-Long Zhang
mouse	WB	Sci Rep; Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice.; Authors - Michael Rabenstein

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph = 6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina and brain at 5dpf

GLAST Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina and brain at 5dpf (Cat no:20785-1-AP)

FH

Ryan (Verified Customer) (01-24-2018)

Antigen retrieval - 0.1M Tris-HCl ph9

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina/brain

GLAST Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish retina/brain (Cat no:20785-1-AP)