GLAST/EAAT1 Recombinant antibody

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  IF Staining of Neuro-2a using 84497-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using GLAST antibody (84497-4-RR, Clone: 241907H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HEK-293 using 84497-4-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GLAST Recombinant antibody (84497-4-RR, Clone:241907H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-937 using 84497-4-RR

  1x10^6 U-937 cells were intracellularly stained with 0.25 ug GLAST Recombinant antibody (84497-4-RR, Clone:241907H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GLAST/EAAT1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Cat No. 84497-4-RR

Clone No.241907H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

GLAST, EAAT1, Sodium-dependent glutamate/aspartate transporter 1, SLC1A3, GLAST-1

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Tested Applications

• Positive IF/ICC detected in: Neuro-2a cells
• Positive FC (Intra) detected in: HEK-293 cells, U-937 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84497-4-RR targets GLAST/EAAT1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GLAST/EAAT1 fusion protein Ag16962
• Full Name: solute carrier family 1 (glial high affinity glutamate transporter), member 3
• Calculated Molecular Weight: 542 aa, 60 kDa
• GenBank Accession Number: BC037310
• Gene Symbol: GLAST
• Gene ID (NCBI): 6507
• RRID: AB_3672010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P43003
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)