GIMAP5 Monoclonal antibody, PBS Only

GIMAP5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6F2

Cat no : 66943-1-PBS

Synonyms

FLJ11296, GIMAP5, GTPase IMAP family member 5, GTPase, IMAP family member 5, hIAN5, HIMAP3, IAN 5, IAN4, IAN4L1, IAN5, IMAP3, Immunity associated protein 3

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 66943-1-Ig (same clone as 66943-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66943-1-Ig (GIMAP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66943-1-Ig (same clone as 66943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66943-1-Ig (GIMAP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66943-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66943-1-Ig (same clone as 66943-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66943-1-Ig (GIMAP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66943-1-PBS in a different storage buffer formulation.

---

Product Information

66943-1-PBS targets GIMAP5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GIMAP5 fusion protein Ag18045
• Full Name: GTPase, IMAP family member 5
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC011732
• Gene Symbol: GIMAP5
• Gene ID (NCBI): 55340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GIMAP5 gene encodes immunity-associated nucleotide 5 protein (IAN5) belonging to GTPase immune-associated protein subfamily. GIMAP5 is widely expressed with high levels in lymph node and spleen. The loss of Gimap5 (GTPase of the 5) gene function is the underlying cause of lymphopenia and autoimmune diabetes in the BioBreeding (BB) rat. Mouse gimap5 is necessary for the survival of peripheral T cells, NK/NKT-cell development, hematopoietic progenitor cell survival and the maintenance of normal liver function. GIMAP5 may regulate T lymphocyte survival by mechanisms that operate upstream of mitochondria.