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GIMAP5 Monoclonal antibody

GIMAP5 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B6F2

Cat no : 66943-1-Ig

Synonyms

FLJ11296, GIMAP5, GTPase IMAP family member 5, GTPase, IMAP family member 5, hIAN5, HIMAP3, IAN 5, IAN4, IAN4L1, IAN5, IMAP3, Immunity associated protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GIMAP5 Monoclonal antibody (66943-1-Ig)

    WB analysis of Jurkat using 66943-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66943-1-Ig (GIMAP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using GIMAP5 Monoclonal antibody (66943-1-Ig)

    IHC staining of human lymphoma using 66943-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66943-1-Ig (GIMAP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using GIMAP5 Monoclonal antibody (66943-1-Ig)

    IHC staining of human lymphoma using 66943-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66943-1-Ig (GIMAP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66943-1-Ig targets GIMAP5 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GIMAP5 fusion protein Ag18045
Full Name GTPase, IMAP family member 5
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC011732
Gene Symbol GIMAP5
Gene ID (NCBI) 55340
RRIDAB_2882267
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GIMAP5 gene encodes immunity-associated nucleotide 5 protein (IAN5) belonging to GTPase immune-associated protein subfamily. GIMAP5 is widely expressed with high levels in lymph node and spleen. The loss of Gimap5 (GTPase of the 5) gene function is the underlying cause of lymphopenia and autoimmune diabetes in the BioBreeding (BB) rat. Mouse gimap5 is necessary for the survival of peripheral T cells, NK/NKT-cell development, hematopoietic progenitor cell survival and the maintenance of normal liver function. GIMAP5 may regulate T lymphocyte survival by mechanisms that operate upstream of mitochondria.

Protocols

Product Specific Protocols
WB protocol for GIMAP5 antibody 66943-1-IgDownload protocol
IHC protocol for GIMAP5 antibody 66943-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols