Anti- GGGGS Linker Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98262-1-RR

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 98262-1-RR

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed

• 

  FC experiment of Transfected HEK-293T using 98262-1-RR

  1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed

GGGGS Linker Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98262-1-RR

Clone No.242306C8

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC

Synonyms

G4S, G4S link, G4S Linker, GGGGS, GS Linker

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Tested Applications

• Positive FC detected in: Transfected HEK-293T cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98262-1-RR targets GGGGS Linker in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: GGGGS Linker
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody was raised against a synthetic peptide (GGGGSGGGGSGGGGS).