CoraLite®532-conjugated GFAP Recombinant antibody

GFAP Uni-rAb^TM Recombinant Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF-P

Conjugate

CoraLite®532 Fluorescent Dye

CloneNo.

4C6

Cat no : CL532-81063

Synonyms

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Validation Data Gallery

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  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6 ) at dilution of 1:800. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL532-81063 targets GFAP in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GFAP fusion protein Ag10423
• Full Name: glial fibrillary acidic protein
• Calculated Molecular Weight: 432 aa, 50 kDa
• GenBank Accession Number: BC013596
• Gene Symbol: GFAP
• Gene ID (NCBI): 2670
• Conjugate: CoraLite®532 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 537 nm / 560 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.