CoraLite®532-conjugated GFAP Recombinant monoclonal antibody

• 

  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6 ) at dilution of 1:800. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, golden ) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, red), CoraLite® Plus 488 MBP antibody (CL488-10458, green), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, golden ) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, red), CoraLite® Plus 488 MBP antibody (CL488-10458, green), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GFAP antibodies are commonly used as specific markers for astrocytes, enabling identification and analysis of glial cells in the central nervous system for neuroscience and pathology research. Proteintech has an extensive range of GFAP antibodies validated for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA, Dot blot and more.

Cat No. CL532-81063

Clone No.4C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF-P

Synonyms

4C6, glial fibrillary acidic protein

---

Tested Applications

• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL532-81063 targets GFAP in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag10423

  Product name: Recombinant human GFAP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 83-432 aa of BC013596

  Sequence: YIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

• Full Name: glial fibrillary acidic protein
• Calculated Molecular Weight: 432 aa, 50 kDa
• GenBank Accession Number: BC013596
• Gene Symbol: GFAP
• Gene ID (NCBI): 2670
• Conjugate: CoraLite®532 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 537 nm / 560 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14136
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.