GFAP Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GFAP Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16825-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of U-251 using 16825-1-AP (same clone as 16825-1-PBS)

  WB result of GFAP antibody (16825-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells. This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

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  WB analysis using 16825-1-AP (same clone as 16825-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16825-1-AP (GFAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

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  IHC staining of Alzheimer mouse using 16825-1-AP (same clone as 16825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Hippocampus tissue slide from Alzheimer Mouse Model using 16825-1-AP (GFAP antibody) at dilution of 1:50000 (under 10x lens). Data from NeuroScience Associates, Inc. This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16825-1-AP (same clone as 16825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16825-1-AP (same clone as 16825-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 16825-1-AP (same clone as 16825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 16825-1-AP (same clone as 16825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 16825-1-AP (same clone as 16825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 16825-1-AP (GFAP antibody) at dilution of 1:1000 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 16825-1-AP (same clone as 16825-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 16825-1-PBS in a different storage buffer formulation.

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Product Information

16825-1-PBS targets GFAP in WB, IHC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GFAP fusion protein Ag10423
• Full Name: glial fibrillary acidic protein
• Calculated Molecular Weight: 432 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC013596
• Gene Symbol: GFAP
• Gene ID (NCBI): 2670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.