GEN1 Monoclonal antibody, PBS Only

GEN1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A10D10

Cat no : 68060-1-PBS

Synonyms

DKFZp781F0986, FLJ40869, Gen, GEN1

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Validation Data Gallery

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  WB analysis of U2OS using 68060-1-Ig (same clone as 68060-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68060-1-Ig (same clone as 68060-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68060-1-Ig (same clone as 68060-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68060-1-Ig (GEN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68060-1-PBS in a different storage buffer formulation.

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Product Information

68060-1-PBS targets GEN1 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GEN1 fusion protein Ag31247
• Full Name: Gen homolog 1, endonuclease (Drosophila)
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 98-100 kDa
• GenBank Accession Number: NM_182625
• Gene Symbol: GEN1
• Gene ID (NCBI): 348654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.