GEMIN5 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 84171-3-RR (same clone as 84171-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84171-3-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84171-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84171-3-RR (same clone as 84171-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-3-RR, Clone: 241205D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84171-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01070-1

  Cytometric bead array standard curve of MP01070-1, GEMIN5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84171-1-PBS. Detection antibody: 84171-3-PBS. Standard: Ag19220. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01070-3

  Cytometric bead array standard curve of MP01070-3, GEMIN5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84171-4-PBS. Detection antibody: 84171-3-PBS. Standard: Ag19220. Range: 0.781-100 ng/mL.

GEMIN5 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 84171-3-PBS

Clone No.241205D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Gem-associated protein 5, Gem associated protein 5, 241205D8

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Product Information

84171-3-PBS targets GEMIN5 as part of a matched antibody pair:

MP01070-1: 84171-1-PBS capture and 84171-3-PBS detection (validated in Cytometric bead array)

MP01070-3: 84171-4-PBS capture and 84171-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GEMIN5 fusion protein Ag19220
• Full Name: gem (nuclear organelle) associated protein 5
• Calculated Molecular Weight: 1508 aa, 169 kDa
• GenBank Accession Number: BC113614
• Gene Symbol: GEMIN5
• Gene ID (NCBI): 25929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TEQ6
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.