GEFT Monoclonal antibody, PBS Only

GEFT Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A11F8

Cat no : 66012-1-PBS

Synonyms

ARHGEF25, GEFT, p63RhoGEF, RhoA/RAC/CDC42 exchange factor

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Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat skeletal muscle using 66012-1-Ig (same clone as 66012-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66012-1-Ig (same clone as 66012-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66012-1-Ig (same clone as 66012-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66012-1-Ig (same clone as 66012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 66012-1-Ig (same clone as 66012-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66012-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66012-1-PBS targets GEFT in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GEFT fusion protein Ag10819
• Full Name: RhoA/RAC/CDC42 exchange factor
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 53 kDa, 63 kDa
• GenBank Accession Number: BC012860
• Gene Symbol: GEFT
• Gene ID (NCBI): 115557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).