CoraLite® Plus 647-conjugated GDI2 Monoclonal antibody

GDI2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4B8F3

Cat no : CL647-60078

Synonyms

FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-60078 targets GDI2 in IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GDI2 fusion protein Ag4613
Full Name GDP dissociation inhibitor 2
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC005145
Gene Symbol GDI2
Gene ID (NCBI) 2665
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 GDI2 antibody CL647-60078Download protocol
Standard Protocols
Click here to view our Standard Protocols