Filter: AllWBIHCIF

WB analysis using 60078-1-Ig (same clone as 60078-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 60078-1-Ig (same clone as 60078-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 60078-1-Ig (GDI2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 60078-1-Ig (same clone as 60078-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 60078-1-Ig (same clone as 60078-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 60078-1-Ig (same clone as 60078-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60078-1-Ig (GDI2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 60078-1-Ig (GDI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GDI2 antibody (60078-1-Ig, Clone: 4B8F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 60078-1-Ig (same clone as 60078-1-PBS)
Immunofluorescent analysis of Hela cells, using GDI2 antibody 60078-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60078-1-PBS in a different storage buffer formulation.

GDI2 Monoclonal antibody, PBS Only

GDI2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 60078-1-PBS

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FLJ16452, FLJ37352, GDI 2, GDI2, GDP dissociation inhibitor 2, Rab GDI beta, RABGDIB

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60078-1-PBS targets GDI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	GDI2 fusion protein Ag4613 Predict reactive species
Full Name	GDP dissociation inhibitor 2
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	46 kDa
GenBank Accession Number	BC005145
Gene Symbol	GDI2
Gene ID (NCBI)	2665
RRID	AB_2232457
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P50395
Storage Buffer	PBS only
Storage Conditions	Store at -80°C.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.