CoraLite®594-conjugated GDI1 Monoclonal antibody

GDI1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4E1G1

Cat no : CL594-66434

Synonyms

FLJ41411, GDI 1, GDI1, GDIL, GDP dissociation inhibitor 1, MRX41, MRX48, Oligophrenin 2, OPHN2, Protein XAP 4, Rab GDI alpha, RABGD1A, RABGDIA, XAP 4, XAP4

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Validation Data Gallery

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  IF Staining of MCF-7 using CL594-66434

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CL594-66434 (GDI1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66434 targets GDI1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GDI1 fusion protein Ag17511
• Full Name: GDP dissociation inhibitor 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC000317
• Gene Symbol: GDI1
• Gene ID (NCBI): 2664
• RRID: AB_2883582
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family. GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI1 is expressed primarily in neural and sensory tissues and also in secretory cells, displaying a diffuse, cytoplasmic distribution in cells. It runs as a 55 kDa protein in SDS-PAGE. (PMID: 7929030,PMID: 19570034)