GDF8/Myostatin Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

GDF8/Myostatin Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19142-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 19142-1-AP (same clone as 19142-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 19142-1-AP (same clone as 19142-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 19142-1-AP (same clone as 19142-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 19142-1-AP (same clone as 19142-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19142-1-AP (GDF8/Myostatin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19142-1-AP (same clone as 19142-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19142-1-AP (GDF8/Myostatin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19142-1-PBS in a different storage buffer formulation.

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Product Information

19142-1-PBS targets GDF8/Myostatin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GDF8/Myostatin fusion protein Ag13605
• Full Name: myostatin
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 50 and 35 kDa
• GenBank Accession Number: BC074757
• Gene Symbol: MSTN
• Gene ID (NCBI): 2660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Myostatin, also known as GDF-8, is a member of the TGF-beta superfamily. Myostatin is expressed in the myotome and developing skeletal muscles. Myostatin negatively regulates skeletal muscle growth and development through the regulation of anabolic and catabolic pathways in skeletal muscles. Myostatin immunoreactive bands could be identified at ∼50, 32-35, and 16 kDa, which correspond to the precursor, glycosylated dimmer, and monomer, respectively (PMID: 17711997).