GDF10 Monoclonal antibody

GDF10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B7D12

Cat no : 66371-1-Ig

Synonyms

BIP, BMP 3b, BMP3B, Bone inducing protein, Bone morphogenetic protein 3B, GDF 10, GDF10

---

Validation Data Gallery

• 

  WB analysis of pig bone marrow using 66371-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 66371-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66371-1-Ig (GDF10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: pig bone marrow tissue, ROS1728 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66371-1-Ig targets GDF10 in WB, ELISA applications and shows reactivity with human, pig, rat samples.

• Tested Reactivity: human, pig, rat
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GDF10 fusion protein Ag4906
• Full Name: growth differentiation factor 10
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC028237
• Gene Symbol: GDF10
• Gene ID (NCBI): 2662
• RRID: AB_2881750
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Growth differentiation factor-10 (GDF10), also known as BMP3b, is a member of the transforming growth factor-b (TGF-b) superfamily. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This promotes neural repair after stroke. GDF10 plays a role in head formation and may have multiple roles in skeletal morphogenesis. Additionally, GDF10 may act as a tumor suppressor and reduced expression of this gene is associated with oral cancer.

Publications

Species	Application	Title
rat	WB	Brain Behav Immun; Schisantherin A attenuates ischemia/reperfusion-induced neuronal injury in rats via regulation of TLR4 and C5aR1 signaling pathways.; Authors - Yun Wei Shi