GDF-15 Recombinant antibody, PBS Only

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  WB analysis using 85100-1-RR (same clone as 85100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85100-1-RR (GDF15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 85100-1-RR (same clone as 85100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85100-1-RR (GDF-15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 85100-1-RR (same clone as 85100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85100-1-RR (GDF-15 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 85100-1-RR (same clone as 85100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85100-1-RR (GDF-15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85100-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85100-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85100-1-RR against Human GDF-15 were performed. The affinity constant is 39.3 nM.

GDF-15 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85100-1-PBS

Clone No.242173B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

GDF15, MIC-1, Growth/differentiation factor 15, growth differentiation factor 15, GDF 15

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Product Information

85100-1-PBS targets GDF-15 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GDF-15 fusion protein Ag26790
• Full Name: growth differentiation factor 15
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC008962
• Gene Symbol: GDF15
• Gene ID (NCBI): 9518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q99988
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. GDF15 has been suggested as a target and biomarker for cardiovascular disease as it plays a cardioprotective role in the adult heart. GDF15 is highly expressed in the placenta. GDF15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy. GDF15 is also produced by adipocytes in response to oxidative stress, a well-known factor associated with preeclampsia.