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GCNA Recombinant antibody, PBS Only

GCNA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241428A9

Cat no : 84124-4-PBS

Synonyms

ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCNA Recombinant antibody (84124-4-RR)

    WB analysis using 84124-4-RR (same clone as 84124-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84124-4-RR (GCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84124-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GCNA Recombinant antibody (84124-4-RR)

    IF Staining of LNCaP using 84124-4-RR (same clone as 84124-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using GCNA antibody (84124-4-RR, Clone: 241428A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84124-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84124-4-RR

    Affinity and Kinetic Characterization of 84124-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84124-4-RR against Human GCNA were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 84124-4-PBS is GCNA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCNA fusion protein Ag21895
Full Name acidic repeat containing
Calculated Molecular Weight 691 aa, 76 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC136259
Gene Symbol GCNA
Gene ID (NCBI) 93953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).