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GCNA Recombinant antibody

GCNA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241428A9

Cat no : 84124-4-RR

Synonyms

ACRC, Germ cell nuclear antigen, Germ cell nuclear acidic protein, Germ cell nuclear acidic peptidase, Acidic repeat-containing protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCNA Recombinant antibody (84124-4-RR)

    WB analysis using 84124-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84124-4-RR (GCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using GCNA Recombinant antibody (84124-4-RR)

    IF Staining of LNCaP using 84124-4-RR

    Immunofluorescent analysis of (4% PFA) fixed LNCaP cells using GCNA antibody (84124-4-RR, Clone: 241428A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84124-4-RR

    Affinity and Kinetic Characterization of 84124-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84124-4-RR against Human GCNA were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inLNCaP cells, DU 145 cells, MCF-7 cells, A549 cells, HepG2 cells, PC-3 cells, human testis tissue
Positive IF/ICC detected inLNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84124-4-RR is GCNA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GCNA fusion protein Ag21895
Full Name acidic repeat containing
Calculated Molecular Weight 691 aa, 76 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC136259
Gene Symbol GCNA
Gene ID (NCBI) 93953
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCNA, also known as ACRC, belongs to the serine-aspartate repeat-containing protein (SDr) family. GCNA has remained tightly associated with sexual reproduction showing enriched expression within germ cells in both invertebrate and vertebrate species. GCNA has recently been implicated in resolving DPCs in germ cells and early embryos. GCNA is targeted to SUMOylated DPC proteins through its SUMO-interacting motifs (SIMs), reinforcing the importance of SUMOylation in DPC repair. The amino acid sequence homology of GCNA in humans and mice is very low. The calculated molecular weight of GCNA is 76 kDa. Sometimes higher molecular weight around 90 kDa can also be observed, which may be caused by SUMO modification (PMID: 31839537, 32329115).

Protocols

Product Specific Protocols
WB protocol for GCNA antibody 84124-4-RRDownload protocol
IF protocol for GCNA antibody 84124-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols