CoraLite®594-conjugated GCK Monoclonal antibody

GCK Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C3A3

Cat no : CL594-67216

Synonyms

GCK, GK, GLK, Glucokinase, glucokinase (hexokinase 4), Hexokinase 4, Hexokinase D, Hexokinase type IV, HHF3, HK IV, HK4, HKIV, HXKP, MODY2

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-67216

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 GCK antibody (CL594-67216, Clone: 1C3A3 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL594-67216

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 GCK antibody (CL594-67216, Clone: 1C3A3 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67216 targets GCK in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GCK fusion protein Ag8116
• Full Name: glucokinase (hexokinase 4)
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001890
• Gene Symbol: GCK
• Gene ID (NCBI): 2645
• RRID: AB_2920098
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glucokinase (GCK) is a structurally and functionally unique member of hexokinase family. It is expressed only in mammalian liver and pancreatic islet beta cells. Because of its unique functional characteristics, the enzyme plays an important regulatory role in glucose metabolism.The rate of glucose metabolism in liver and pancreas is a function of the activity of the enzyme(PMID:1740341). Moreover, GCK has been found to have relationship with diabetes. Defects in GCK are the cause of maturity-onset diabetes of the young type 2 (MODY2) and familial hyperinsulinemic hypoglycemia type 3 (HHF3). It has 3 isoforms produced by alternative splicing with the same molecular mass of 52 kDa.